Tuesday, June 18, 2013

NUTRIENT MEDIA AND HORMONES IN PLANT TISSUE CULTURE

assalammualaikum w.b.t and have nice .

i'm going to take my exam tommoroww about tissue culture. hope everthing will run smoothly. ya ALLAH please helps me and easy for to answer the question.



yes!umk i'm stay with you^^



::DEFINITION OF MEDIUM ::
1. when you make an explant like an axillary buds ,you remove it from the sources of many chemicals and have to resupply these to the explants to allow them to grow.
2. a nutritrive solution ,solid or liquid used for culturing plant cells and tissue.

shoots= auxin and gibbrelins
roots=cytokinin

::F(X) OF MEDIUM::
provide
1. water
2.mineral nutritioons
3.vitamins
4.growth regulators
5.acces to atmosphere for gas exchange
6.removal of plants metabolites waste.

::MAJOR CONSTITUENTS::
1.inorganic salts
-macro elements
-micro elements
2.organic constitents
-carbon source 
-vitamins
-hormone
3.gelling agent
4.other additive
5water

::F(x) OF MACRO AND MICRO ELEMENTS::

1.NITROGEN  = DNA/proteins/chloropyll/amino acids/hormones
2.PHOSPHORUS= abundant in meristematic and fast growing tissue / essential for photosynthesis 
3.POTASSIUM (k) = necessary for cell divison ,meristematic tissue / helps in the pathway for carbohydrates(k) ,protein and chloropyll(k) synthesis.
4. CALCIUM =  involved in formation of cell wall and roots and leaf developments / translocations sugar and amino acids
5. IRON = respirations ,chloropyll and photosynthesis.
6.MAGNESIUM = involved in photosynthetic and respiratory systemic / active in uptake phosphate and translocations of phosphate and straches
7. SULPHUR= formation of nodules/ and chloropyll synthesis / structural components of amino acids and enzyme.
8.MANGANESE= involved in regulation of enzyme and growth hormones / assits in phitisynthesis and respiration
9.MOLYBDENUM = involved in enzymatic reduction of nitrates to ammonia / 
10. ZINC= involved inn priduction of growth hormones and chloropyll  / respiration and carbohydrates synthesis.
11. BORON = involved in production growth hormone  and chloropylll / active in respiration and carbohyrates synthesis
12.COOPER = invoolved in photosynthetic and respiration system/ used as a catalyst reaction.

rehat kejap
*yang jauh aku nampak kecik..begitu juga study ,bagitau pada hati bahawa semua akan jadi senang jika minta tolong dengan ALLAH


::TYPES AND FORMULATIONS OF BASAL MEDIA ::

1.MS MEDIUM==>> whole plant regenerations
2.B5 MEDIUM ==>>legumes (soyabeans culture) /calllus suspesion /less nitrate and ammonium than MS
3.WHITE MEDIUM==>>for tomatoes  and less salts than MS
4.NITSCH& NITSCH MEDIUM ( 1969)==>>anther culture / MS =salt=WHITE
5.WOODY PLANTS MEDIUM ( WPM) / HILDER BRANDT  (SH) MEDIUM 1972==>>woody plants 


::VITAMINS::



commonly used
1.inositol = part of B complex ,cell membrane organelles
2.thiamine = vitamin B1 a coenzyme in the citric acid cycle
3.pyridoxine: vitamin B6

AMINO ACIDS
1.Tyrosine = stimulates morphogenesis in cell culture

2.Adenine= stimulates cell growth and greatly enhances shoot formation , Part of RNA and DNA.

GELLING AGENTS / SUPPORT
1. agar
2.agarose
3.geirite
4.phytagar
5.mechanical
6.sand
7.filter paper
8.glass wool
9.silica gel
10.starch /pectin.

MEDIA PREPARATION
1. Stock solutions (macro +micro+vtamins+hormones)
2,adjust volume to 800mL
3.check pH (5.4-5.8) made upto 1L add 30g sucrose ,8 g agar.
4.dispense into culture vessels
5.autoclave the medium 


yes!alhamdulillah !finish=)
then..
lest we revise back.
1.definition of medium
2.function of medium
3.function of macro and micro elements
4.formulations of basal media
5. vtamins and amino acid and gelling agents
6 .media preparations
=)

that all.
DOAKAN KEJAYAAN SUPERWOMAN AGRICULTURE DIRECTOR^^



SEKIAN.ASSALAMMUALAIKUM W.B.T DAN SALAM KEBAHAGIAAN.







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